FastQCFastQC Report
Thu 13 Sep 2018
HGTGFBGX7_n01_df5174rnaseq.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTGFBGX7_n01_df5174rnaseq.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51799375
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACC332600.015.258611145
AAGAGCC356650.012.176537145
CGGGCTA31700.011.8935131
CCCGGGT58400.011.5461211
CCCGGGA82100.011.4806431
CCGGGAT145200.011.4349661
CCAGTCC241500.011.167732145
CCCGGCT79200.010.7109181
GGGCACT120850.010.3192431
GCCGGTT218350.010.1277441
CCCGTAT113900.010.1213731
CCGGGTT165800.010.0142161
GGGGAAT212050.09.8131781
CCCTGGT95200.09.5962091
GGGGTCT81100.09.4765671
CCCGGAT159200.09.427431
CGGGGCT58950.09.3475071
CCCCCAT112000.09.1925571
CGGGAAA347500.08.9509371
GCCGTAT155550.08.9494691
Produced by FastQC (version 0.11.2)