FastQCFastQC Report
Thu 13 Sep 2018
HGTGFBGX7_n01_df5173_rnaseq.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTGFBGX7_n01_df5173_rnaseq.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46933086
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGTCC151750.013.234311145
GGGGCCT28250.012.5763421
CCCCGGT40700.012.4703981
CCCGGCT107950.012.0900071
GAGCACC446850.011.941707145
CGGGCAT177500.011.7235791
ATGCCGG143800.010.134154145
CCCGGGT124850.010.1050241
CCCGGTA46000.09.9302321
CGGGGCT50800.09.8483181
TGCCGTG99450.09.8419075145
CCCTGGT66750.09.7761521
GCCCTAT14601.193257E-99.4357721
CCCGGAT181200.09.4034321
TATGCCG447000.09.374986145
CCCCGTT72850.09.3556731
CGGGGTA43450.09.1780331
CCCGTAT84650.09.1650221
GTATGCC500000.09.149758145
CCCGGGA54150.08.97125151
Produced by FastQC (version 0.11.2)