FastQCFastQC Report
Thu 13 Sep 2018
HGTGFBGX7_n01_df5112_rnaseq.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTGFBGX7_n01_df5112_rnaseq.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37789545
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT383000.1013507836625183No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGCTA39850.019.103428145
CCCCTGT19100.017.8413411
CCCGGGC22500.017.4010031
CCCCGGG26450.015.3505991
CCCGGGT35200.015.2423531
GAGCACC269900.015.177349145
CCGGGAT84150.013.8718611
CCGGCCT87550.013.6644071
CCCCGGT35750.013.1825761
CCGGGGA38250.012.889631
GCGGGCT60950.012.8473351
CCTATGG27750.012.7974238
CCCGGGA25000.012.7607351
CCCTGGT93500.012.6397651
AGTCACC121800.012.619407145
TGCCGTG76350.012.534738145
AGGCTAC43400.012.3621025145
GCGGGAT96850.012.3522841
CCCCGAT49950.012.1928941
GGGGAAT109600.012.1722341
Produced by FastQC (version 0.11.2)