FastQCFastQC Report
Thu 13 Sep 2018
HGTGFBGX7_n01_df5070_rnaseq.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTGFBGX7_n01_df5070_rnaseq.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47201741
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT740440.15686709522006825No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGTCC147500.018.924349145
CCCCGGT31100.017.2517191
CCGGCCT91950.015.9279781
CCGGGAT87950.015.0860751
CCCGGGA21550.014.8035691
CCCCTGT20650.014.3954351
CGGGCAT86050.013.9867951
CCCGTGT55600.013.8226751
GAGCACC348750.013.512989145
GCGGGCT63200.013.19294551
GCCCTAT17850.012.5917041
CCCTGGT77950.012.3707651
CCCCGTT51200.012.3199891
GCCGGTT150700.012.1240651
GTGCCCT66750.011.92278920-24
CGCGGGT23100.011.613171
CCCGGTA39500.011.5639041
CACGCCT70000.011.51351210-14
CGGGGCT42850.011.505861
CGCCCTA12103.8198777E-1111.3849011
Produced by FastQC (version 0.11.2)