FastQCFastQC Report
Thu 13 Sep 2018
HGTGFBGX7_n01_df5025_rnaseq.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTGFBGX7_n01_df5025_rnaseq.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39353076
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACC456200.015.256821145
AAGAGCC395450.013.732142145
CTTTATT262250.012.5515961
GTTTATT267750.012.5103961
TTAGGCC131450.010.8656145
TTTATTC237900.010.14759352
AGGCATG399700.09.776935145
GGCAAAT309450.09.161041
TTAACGC87800.09.080487
TCACTTA1518050.09.069575145
ATGCCGG147050.08.973346145
CGTATGC563350.08.9316145
GCCGGAT150150.08.8365821
GTATGCC525850.08.824014145
TATGCCG461650.08.763335145
TTTATTT417050.08.5524452
TCGTATG593450.08.5518875145
GTCACTT1660450.08.462059145
TTTTATT389550.08.4304032
ACTTAGG1367150.08.400151145
Produced by FastQC (version 0.11.2)