FastQCFastQC Report
Thu 13 Sep 2018
HGTGFBGX7_n01_df5022_rnaseq.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTGFBGX7_n01_df5022_rnaseq.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46640550
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACC612400.013.425359145
ATGCCGG172100.012.638292145
ATGTATG363700.011.82114145
AAGAGCC538900.011.005077145
TGTATCG300150.010.579953145
TATGCCG627050.09.504246145
CCAGTCC434750.09.105442145
GCCGGAT176800.09.022021
CGGGTAT123550.08.8026181
TATCTCG901800.08.755192145
TGCCGTG178700.08.72292145
GTATGCC772800.08.612388145
CCCGGCT130250.08.5168121
CCGATGT1881850.08.418107145
ACCGATG1930150.08.350191145
GCCGTTT247200.08.3004451
CACCGAT2125800.08.270607145
GCCGTAT145100.08.1948271
GGCAAAT604450.08.15665151
TCGTATG858400.08.133633145
Produced by FastQC (version 0.11.2)