FastQCFastQC Report
Thu 13 Sep 2018
HGTGFBGX7_n01_df5020_rnaseq.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTGFBGX7_n01_df5020_rnaseq.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46011248
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1562990.3396973713905782No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCATT417450.015.7329216
TTATAAC155800.015.2616823
GTTTATT401200.014.5838551
AATTCAT386000.014.3668835
GAGCACC622300.014.201798145
CGGTTTA144700.013.8772364
TCACTGC393200.013.847323145
CTTTATT406550.013.7677531
AAGAGCC551400.013.450806145
TTTTTAA685500.012.2796081
TTTTAAC278350.011.8503792
GGTTTAT205450.011.5379875
TTTTTTA815300.011.187241
CGCGAAA183950.010.87851
GTTTTAT265050.010.5589131
TTTTAAT366150.010.5344611
CAATTCA325600.010.3751244
TTTATTC433300.010.0553652
TTTAATG153200.09.7007912
TTTTAAA618200.09.6757511
Produced by FastQC (version 0.11.2)