FastQCFastQC Report
Thu 13 Sep 2018
HGTGFBGX7_n01_df5010_rna_seq.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTGFBGX7_n01_df5010_rna_seq.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42306392
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGGGT55450.012.9446551
GAGCACC639900.012.508629145
CACGATG459100.012.144281145
TATGCCG634550.011.814284145
GTATGCC711350.011.639532145
AAGAGCC548200.011.334295145
CGTATGC816750.010.909767145
CCAGTCC305300.010.876556145
TCGTATG950650.010.212044145
CCCGGAT102350.09.8465431
CACACGG184650.09.619877145
ATCACGC347000.09.5694895145
GCCGTCT673350.08.926196145
CTCGTAT951750.08.836654145
ACATCAC2220800.08.563308145
AGTCACA2685250.08.499683145
CAGTCAC2879300.08.372545145
CCCGGGA79800.08.2679031
GTCACAT2531550.08.245328145
GCCGGTT182200.08.23718551
Produced by FastQC (version 0.11.2)