FastQCFastQC Report
Thu 13 Sep 2018
HGTFWBGX7_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTFWBGX7_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31943151
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1583880.4958433812619175No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG22900.019.4313871
TACCCGC9450.014.81676823
ACCGATC17900.014.27403734
CGAGACC18950.013.66898330
CCGAGAC20150.013.37798229
GCATTCC13100.012.82449153
CCCGCTG11200.012.50286125
TTACCCG10850.012.2575322
ACCCGCT11350.012.02882824
GAGACCG21350.011.96800331
CGGGATT11450.011.91957217
TCAGGCG10950.011.50501512
GCGGGAT10850.011.28855916
AGACCGA22100.011.24466432
GATTACC11100.011.03514120
CAGGTCA11850.010.9264438
GGAATGC26100.010.72938323
CCCAGGT8350.010.47829156
CGGTTCA27450.010.19870713
ATAAGCG8600.010.17400847
Produced by FastQC (version 0.11.5)