FastQCFastQC Report
Thu 13 Sep 2018
HGTFWBGX7_n01_pIXHALO_col_wg-a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTFWBGX7_n01_pIXHALO_col_wg-a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences930523
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT33740.3625917897784364TruSeq Adapter, Index 13 (97% over 38bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATGCC11590.12455361124872787TruSeq Adapter, Index 13 (97% over 35bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10470.11251736926438143No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT6350.039.6838851
TGCCGTC6950.036.76151750
ATGCCGT6950.036.25793549
TATGCCG7000.035.99894748
GTATGCC7500.033.59902247
CCGTCTT8100.031.11020752
CGTATGC8300.030.3605646
CTTCTGC9000.028.38806356
CTGCTTG8900.028.31378259
CGTCTTC9250.027.62081553
TGCTTGA10200.025.04828860
TTCTGCT10450.024.78396657
GCTTGAA10700.024.53199661
TCTGCTT10600.024.43324958
TCGTATG10350.024.34711645
AAAGGGG4200.023.33390870
CTCGTAT10950.023.33265144
TCTTCTG12050.021.20270255
TCTCGTA13100.020.03758243
CTTGAAA13250.019.01831262
Produced by FastQC (version 0.11.5)