FastQCFastQC Report
Thu 13 Sep 2018
HGTFWBGX7_n01_pIXHALO_col_rr-a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTFWBGX7_n01_pIXHALO_col_rr-a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1086929
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT46540.42817884148826646TruSeq Adapter, Index 27 (97% over 39bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCC15750.1449036689608981TruSeq Adapter, Index 27 (97% over 36bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11710.107734727843309No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT8050.041.7374351
TATGCCG8000.041.56080648
TGCCGTC8050.041.30266650
ATGCCGT8250.040.30138849
GTATGCC8800.037.7825547
CGTATGC9550.034.8153446
CCGTCTT9900.034.2931152
CTGCTTG10000.033.60017859
CTTCTGC11650.029.14312456
CGTCTTC11750.028.29802153
TCGTATG12100.028.05670745
CTCGTAT12100.027.18897444
TGCTTGA13400.025.07475960
TTCTGCT13900.024.67752557
GCTTGAA13650.024.61551361
AAAGGGG4850.024.53621170
TCTGCTT14400.023.82066558
TCTTCTG15300.022.1896655
TCTCGTA15600.021.31323443
CTTGAAA17550.019.54426262
Produced by FastQC (version 0.11.5)