FastQCFastQC Report
Thu 13 Sep 2018
HGTFWBGX7_n01_ZmTCP11_retic_NYU6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTFWBGX7_n01_ZmTCP11_retic_NYU6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1425689
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT63040.44217217078900095TruSeq Adapter, Index 3 (97% over 36bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23080.16188663867084618No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTATGCC21960.15403078792078778TruSeq Adapter, Index 3 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT12900.038.799549
TATGCCG12900.038.52817548
GCCGTCT12900.038.25684751
GTATGCC14700.034.28664447
CCGTCTT14900.033.3566152
TGCCGTC14900.033.3566150
CGTATGC15350.032.60674346
GCTTGAA16000.030.84458561
CTGCTTG16050.030.5304259
TGCTTGA17150.028.98037560
CTTCTGC17500.028.20076456
TTCTGCT17750.028.19794557
CGTCTTC17900.027.9606753
TCGTATG18200.027.50074645
CTCGTAT18650.026.46184244
TCTGCTT19200.026.06841358
TCTTCTG20500.024.07382255
CTTGAAA20750.023.95245662
TCTCGTA22000.022.5915243
AAAGGGG8750.021.20057570
Produced by FastQC (version 0.11.5)