FastQCFastQC Report
Thu 13 Sep 2018
HGTFWBGX7_n01_ZmTB1_retic_NYU4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTFWBGX7_n01_ZmTB1_retic_NYU4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2876915
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT142350.4948008543874254TruSeq Adapter, Index 7 (97% over 35bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATGCC50630.17598712509754372TruSeq Adapter, Index 7 (96% over 32bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43260.15036940611731664No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT24750.043.55352451
ATGCCGT25650.042.29895449
TGCCGTC25700.042.08048250
TATGCCG26750.040.1670548
GTATGCC30000.035.9322847
CCGTCTT30450.035.51619752
CTGCTTG31300.035.33442759
CGTATGC31800.034.00784746
CTTCTGC33900.032.1087656
GCTTGAA36500.029.82104561
TTCTGCT36950.029.55309757
CGTCTTC36800.029.48283453
TCGTATG36800.029.29211245
TGCTTGA37350.029.14238560
TCTGCTT39550.027.7872858
CTCGTAT39050.027.42557344
TCTTCTG43250.025.410155
CTTGAAA45500.024.07663562
TCTCGTA47750.022.57564743
TGAAAAA51950.021.15470364
Produced by FastQC (version 0.11.5)