FastQCFastQC Report
Thu 13 Sep 2018
HGTFWBGX7_n01_ZmLG1_retic_NYU3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTFWBGX7_n01_ZmLG1_retic_NYU3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3108398
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT80180.2579463762362478TruSeq Adapter, Index 13 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG17450.035.298448
GCCGTCT20050.030.54699351
GTATGCC20500.029.70572547
ATGCCGT20700.029.41918849
CGTATGC21100.029.35861646
CCGTCTT21000.029.33223352
TCGTATG24000.025.66529545
TGCCGTC24300.025.0607950
CGTCTTC26900.023.41957553
CTCGTAT26200.023.37661244
CTGCTTG26450.023.02297259
TCTGCTT27100.022.60026758
GCTTGAA27450.022.5663761
TTCTGCT29200.020.85504757
TGCTTGA30400.020.60678760
CTTCTGC30400.020.49232556
TCTTCTG32100.019.95284555
CTTGAAA31950.019.7166262
TCTCGTA32550.019.0318543
AATCTCG36750.016.66711441
Produced by FastQC (version 0.11.5)