FastQCFastQC Report
Thu 13 Sep 2018
HGTFWBGX7_n01_ZmKN1_retic_NYU1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTFWBGX7_n01_ZmKN1_retic_NYU1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2220145
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT111580.5025797864553891TruSeq Adapter, Index 2 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGCC39460.17773613885579545TruSeq Adapter, Index 2 (97% over 34bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30700.13827925653504614No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT21250.041.50477251
TATGCCG21250.041.34100348
ATGCCGT21350.041.1473749
TGCCGTC22100.039.75096550
GTATGCC23900.036.9036147
CCGTCTT24450.036.21580552
CTGCTTG24900.035.7018659
CGTATGC26350.033.73846
CGTCTTC29150.031.45713853
GCTTGAA29350.030.52729261
TCGTATG29650.030.10105345
CTTCTGC29850.029.89869956
TGCTTGA30550.029.09905860
TTCTGCT31400.028.5342757
CTCGTAT32600.027.48454944
TCTGCTT33050.026.89791958
CTTGAAA35350.025.74188662
TCTTCTG35400.025.21119355
TCTCGTA38400.023.24156643
AAAGGGG13800.021.55836570
Produced by FastQC (version 0.11.5)