FastQCFastQC Report
Thu 13 Sep 2018
HGTFWBGX7_n01_ZmHB54_retic_NYU7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTFWBGX7_n01_ZmHB54_retic_NYU7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1738225
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT46220.2659034359763552TruSeq Adapter, Index 7 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT8950.039.88754751
TGCCGTC9700.036.44264250
ATGCCGT10050.035.52174849
TATGCCG10200.034.99936748
CCGTCTT11350.031.1448152
CGTATGC11900.029.99945846
GTATGCC12350.028.90635947
CTGCTTG13050.027.08763359
TCGTATG14100.025.31869345
CTCGTAT14600.023.9721744
CTTCTGC14900.023.959356
CGTCTTC15100.023.87374153
GCTTGAA16350.022.47730461
TTCTGCT16950.021.06156357
TCTGCTT17600.020.28372258
TGCTTGA18700.019.65261760
TCTCGTA18850.018.75297543
TCTTCTG19750.018.07562355
ATCTCGT20850.017.12199442
CTTGAAA21700.016.93566562
Produced by FastQC (version 0.11.5)