FastQCFastQC Report
Thu 13 Sep 2018
HGTFWBGX7_n01_ZmHB117_FBR1_retic_NYU5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTFWBGX7_n01_ZmHB117_FBR1_retic_NYU5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1986435
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT67090.3377407264773325TruSeq Adapter, Index 19 (97% over 38bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATGCC23390.11774863008354162TruSeq Adapter, Index 19 (97% over 35bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20910.10526395275959194No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT11900.037.94125751
ATGCCGT12350.036.5578649
TGCCGTC12550.036.53302450
TATGCCG13100.034.46572548
CCGTCTT14000.032.5000752
GTATGCC15700.028.98095547
CGTATGC16600.027.40969746
CTGCTTG16950.027.2560159
CTTCTGC18100.025.33154556
CGTCTTC18650.024.77217153
GCTTGAA19400.023.99429361
TTCTGCT19650.023.51150157
TCGTATG19850.023.2746145
CTCGTAT21100.021.56402644
TGCTTGA22050.021.11009460
TCTGCTT22400.020.46827958
TCTTCTG23600.019.87292555
TCTCGTA24400.018.64758143
CTTGAAA26450.017.59883962
ATCTCGT28050.016.47103942
Produced by FastQC (version 0.11.5)