FastQCFastQC Report
Thu 13 Sep 2018
HGTFWBGX7_n01_ZmBZIP2_DFL1_retic_NYU8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTFWBGX7_n01_ZmBZIP2_DFL1_retic_NYU8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1630386
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT67790.41579110713659223TruSeq Adapter, Index 6 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTATGCC23500.14413764593169962TruSeq Adapter, Index 6 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16560.10157103900548706No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT11850.040.75811851
ATGCCGT11850.040.4627749
TATGCCG12400.038.950348
TGCCGTC12700.038.30579450
GTATGCC13900.034.99881747
CCGTCTT14300.034.2645852
CGTATGC14350.033.1696146
CTGCTTG15500.030.70864159
TCGTATG17250.027.99819845
GCTTGAA18050.026.56338761
CGTCTTC18450.026.17797953
CTTCTGC19550.025.42191756
TGCTTGA18900.025.18356360
CTCGTAT18950.025.11711744
TTCTGCT20400.024.36192557
TCTGCTT20650.023.55852758
TCTTCTG22700.022.04839155
TCTCGTA22500.021.46528443
CTTGAAA22650.021.16861362
ATCTCGT26750.018.05546642
Produced by FastQC (version 0.11.5)