FastQCFastQC Report
Thu 13 Sep 2018
HGTFWBGX7_n01_ZmBBM_retic_NYU2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTFWBGX7_n01_ZmBBM_retic_NYU2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2629834
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT28810.10955064083892749TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT10700.019.95316949
TATGCCG10400.019.85566748
CGTATGC10650.019.71820846
GTATGCC11700.018.54691347
GCTTGAA12500.017.3592561
TCGTATG11900.017.35285245
CTCGTAT12250.016.57134244
TGCCGTC12900.016.27867750
GCCGTCT15850.013.69052151
TCTCGTA15750.012.888823543
CTTGAAA17700.012.65482162
CTGCTTG16650.012.61206859
TGCTTGA17000.012.35240760
TGAAAAA18350.012.01605964
TTCTGCT19550.011.63721157
ATCTCGT19050.010.655910542
TCTTCTG20350.010.49174555
CTTCTGC23150.010.28111656
GTCTTCT22800.09.82488454
AAAAAAG23050.09.7177770
Produced by FastQC (version 0.11.5)