FastQCFastQC Report
Thu 13 Sep 2018
HGTFWBGX7_n01_MADS41_BECRAFT17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTFWBGX7_n01_MADS41_BECRAFT17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1678708
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT16860.10043438167924379TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC5050.026.33555846
TATGCCG5250.025.33229848
ATGCCGT5400.025.27674749
GCGAAAT5650.024.15974437
CTCGTAT5650.023.53886244
TCGTATG5900.023.13464745
AATCTCG6700.020.89529241
CGCGAAA6550.020.30572336
CCGCGAA6600.020.15189235
GCCGTCT6850.019.92619351
CGAAATC6900.019.7829838
TCTCGTA6950.019.63948443
TGCCGTC7600.018.88080650
ATCTCGT7550.018.5422942
CACTCCG8100.018.1501131
GTATGCC7350.018.094547
CCGTCTT7700.017.72707652
TCCGCGA8700.016.49554434
GTCACTC8400.016.25175729
GCTTGAA9450.015.55491961
Produced by FastQC (version 0.11.5)