FastQCFastQC Report
Thu 13 Sep 2018
HGTFWBGX7_n01_HALO-ZmOS1_ws_retic.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTFWBGX7_n01_HALO-ZmOS1_ws_retic.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2120236
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC4850.020.9274946
CTCGTAT5400.018.1478544
TCGTATG5950.017.64676745
TATGCCG5700.017.19229348
ACGTCTG6000.016.33229415
ATGCCGT6050.016.19769949
CACGTCT6850.014.30565914
ACACGTC6950.014.09982213
CATTCAG7550.013.44443833
GCCGTCT7900.013.290606551
ACTCCAG7600.012.89513123
TGCCGTC8400.012.49979350
TCCAGTC8450.012.42670925
CCAGTCA9350.012.35361226
TCTCGTA7950.012.3265543
CACACGT8300.012.22813712
AATCTCG8550.011.8708741
GTCACAT9350.011.60518229
GTATGCC9200.011.41285547
CCGTCTT8900.011.40430652
Produced by FastQC (version 0.11.5)