FastQCFastQC Report
Thu 13 Sep 2018
HGTFWBGX7_n01_HALO-OSbZIP77_retic.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTFWBGX7_n01_HALO-OSbZIP77_retic.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1276589
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATGCG2900.024.1371934
CTCGTAT2800.022.49930844
AATGCGC3300.020.15326335
ATGCGCA3650.020.13794536
CGTATGC3400.019.55822246
TCGTATG3450.019.27476945
TCTCGTA3850.018.1812643
TGCGCAT4250.018.1185137
GCCGTCT4150.017.71029951
CCGTCTT4050.017.28409652
ATGCCGT3850.017.27151949
CTAATGC4500.017.11058433
CGTCTGA3750.016.79882416
CGCATCT4250.016.47137339
GCGCATC4500.016.3341138
AGTCACT4250.015.6490328
ATCTCGT4500.014.77790342
GTCACTA4403.6379788E-1214.31998429
GTATGCC4750.013.99902247
CAGTCAC5150.013.59394527
Produced by FastQC (version 0.11.5)