FastQCFastQC Report
Thu 13 Sep 2018
HGTFWBGX7_n01_HALO-OSbZIP69_retic.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTFWBGX7_n01_HALO-OSbZIP69_retic.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences632239
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG1253.5579433E-927.99740615
GTATGCC1451.7265847E-824.13760447
CGTCTGA1652.6593625E-923.33117116
ATGCCGT1852.2701533E-718.91716649
CAGTCAC2104.2560714E-818.33598527
CACGTCT1953.9465885E-717.94705614
TATCTCG2005.142192E-717.49976341
TCTCGTA2005.142192E-717.49976343
CGTATGC2056.6596294E-717.07293946
TATGCCG2151.0956228E-616.27884948
CCGTCTT2151.0965286E-616.27756152
AGTCACC2201.389164E-615.91265128
CTCGTAT2201.3926074E-615.908874544
ACACGTC2251.761513E-615.55411513
TCGTATG2302.2124277E-615.21718445
GTCTGAA3051.2090823E-814.91665117
CACACGT2352.7689093E-614.89223712
GCCGTCT2352.7689093E-614.89223751
GCTTGAA2604.855465E-714.8063261
TGCCGTC2604.855465E-714.8063250
Produced by FastQC (version 0.11.5)