FastQCFastQC Report
Thu 13 Sep 2018
HGTFWBGX7_n01_HALO-OSbZIP42_retic.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTFWBGX7_n01_HALO-OSbZIP42_retic.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2148954
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT6550.021.9083944
TCGTATG6300.021.66665845
TCTCGTA6800.021.10195243
CCGTCTT7150.020.55799952
TATGCCG7250.018.8275848
GCTATGA8000.018.37627836
CGGCTAT8200.017.9272434
CCGGCTA8100.017.71728133
GTATGCC7800.017.49999447
GCTTGAA8500.016.46981461
ATCTCGT8850.016.21430642
GGCTATG8650.016.18647235
TATGATC8900.016.12471638
CTTGAAA9050.015.85561162
CTATGAT9300.015.05480937
TGCTTGA10200.014.41075460
GCCGTCT9750.014.35796751
CGTATGC10050.014.27860346
ATGCCGT10050.013.93034449
GATCTCG10600.013.86856541
Produced by FastQC (version 0.11.5)