FastQCFastQC Report
Thu 13 Sep 2018
HGTFWBGX7_n01_HALO-OSZOS_retic.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTFWBGX7_n01_HALO-OSZOS_retic.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences743622
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAA1604.903086E-821.8758436
CGTATGC1656.7937435E-821.2115146
CCGCGAA1801.7001366E-719.44519235
CTCGTAT2002.4396286E-819.24944544
CCGTCTT2451.326589E-817.14236352
TCGTATG2009.956819E-615.74954645
ATGCCGT2503.113255E-715.39955649
GTATGCC2051.2581953E-515.36541147
TCTCGTA2302.214052E-615.21695343
TCCGCGA2151.9728679E-514.65172634
CGAAATC2556.454142E-613.72509438
AGTCACT2952.0131083E-613.05222628
TATGCCG2559.806997E-512.35258548
GTCACTC3154.1971707E-612.22351429
AATCTCG2601.17561125E-412.11503541
TGCCGTC3255.957969E-611.84581250
ACTCCGC3003.4280994E-511.66711532
GCGAAAT2701.6709442E-411.66633137
ATCTCGT3003.4304565E-511.6663342
CACTCCG2852.7586627E-411.05305731
Produced by FastQC (version 0.11.5)