FastQCFastQC Report
Thu 13 Sep 2018
HGTFWBGX7_n01_HALO-NAC12_retic.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTFWBGX7_n01_HALO-NAC12_retic.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1927870
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGGCTAACACCTACGGATTTTTGACCAAGAAATGGTCTCCACCAGAAA33950.17610108565411567No Hit
GGATTTTTGACCAAGAAATGGTCTCCACCAGAAATCCAAGAATGTGATCT27920.1448230430475084No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGC750.001833431423.33343740
TACCGTC850.003791584920.58832670
CAACTGT2750.002545151410.18186366
TGGAGAG8350.010.05992468
GTAGTGG9151.8189894E-129.1801364
GTTGCGG4301.3046453E-48.95352870
TGTTGCG5151.0205681E-48.155375569
GCACACG13101.8189894E-127.480949470
TCTACGA18000.07.3887364
TACGACA17300.07.08095766
GAGAGGA15151.8189894E-126.930723770
CCGTGCT6157.8819634E-46.829121653
ATCTACG19550.06.80292363
ATAACCG6208.642587E-46.77404810
TAGACTT5300.0098933026.60380368
ACCCCGT5300.0098957736.60363250
ACGACAA19250.06.545483667
CTACGAC18750.06.533192665
AGTACAC6100.00541587556.311339456
TTCTCCG9501.2594184E-56.263185570
Produced by FastQC (version 0.11.5)