FastQCFastQC Report
Thu 13 Sep 2018
HGTFWBGX7_n01_GST-OsbZIP69_10ulBeads.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTFWBGX7_n01_GST-OsbZIP69_10ulBeads.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences606816
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCGA1650.031.81713334
CGCGAAA1700.030.88133636
CCGCGAA1750.029.9990135
ACTCCGC1900.029.47271232
CTCGTAT1800.029.16570544
TCTCGTA2000.027.99907943
ACGTCTG1900.027.63066715
GCGAAAT1950.026.9221937
CGTATGC2200.025.45370746
TATGCCG2200.025.45370748
GTATGCC2200.025.45370747
ATGCCGT2200.025.45370749
TCGTATG2100.024.99917445
GTCACTC2300.022.82909829
AGTCACT2500.022.40295428
CGTCTGA2551.8189894E-1220.58755716
CACTCCG2551.8189894E-1220.58755731
CGAAATC2900.020.51656338
ACACGTC2603.6379788E-1220.19164313
CAGTCAC2800.020.00263827
Produced by FastQC (version 0.11.5)