FastQCFastQC Report
Thu 13 Sep 2018
HGTFWBGX7_n01_GST-OsbZIP24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTFWBGX7_n01_GST-OsbZIP24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1089374
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT19920.18285731071239078TruSeq Adapter, Index 8 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC3750.031.7317746
TCGTATG4000.029.74853745
CTCGTAT4050.028.51711744
TCTCGTA4600.025.86829243
TATGCCG5000.023.7988348
CCGTCTT4900.023.57243352
GTATGCC5050.023.56319647
CGCGCAT5550.023.33325837
GCCGTCT5300.021.79138251
ATCTCGT5500.021.635342
GCGCATC6250.021.2799338
ATGCCGT5650.021.06091149
TCGCGCA6000.020.41847436
GCTTGAA5950.019.99901661
ACTCTCG5950.019.41348332
CGCATCT6300.018.88882639
TCTCGCG6550.018.16954834
TGCCGTC6800.016.98445950
CTTGAAA7200.016.04087862
GTCTTCT7650.015.55621754
Produced by FastQC (version 0.11.5)