FastQCFastQC Report
Thu 13 Sep 2018
HGTFWBGX7_n01_GST-OsZOS.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTFWBGX7_n01_GST-OsZOS.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1183138
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT217991.8424731519062019TruSeq Adapter, Index 27 (97% over 39bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC27600.057.1886846
GTATGCC28200.055.97189747
TCGTATG28250.055.87283345
TATGCCG28400.055.70096648
GCCGTCT28550.055.0405651
CCGTCTT28550.054.91797652
CTCGTAT28650.054.23995644
TCTCGTA28900.053.89185343
ATGCCGT29400.053.68733249
GCTTGAA29800.052.73626761
TACTCGA30200.052.1536736
ATCTCGT29900.051.97239742
ACTCGAT30500.051.5237537
TTACTCG30950.050.77676435
TGCCGTC31250.050.28505350
CTTGAAA31400.050.16053462
CTTCTGC31750.049.9361956
GATCTCG31100.049.85449241
TTCTGCT31950.049.40451457
GTCTTCT32500.048.6761354
Produced by FastQC (version 0.11.5)