FastQCFastQC Report
Thu 13 Sep 2018
HGTFWBGX7_n01_EREB130_BECRAFT10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTFWBGX7_n01_EREB130_BECRAFT10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2040320
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT27800.13625313676286072TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC6050.033.55267746
ATGCCGT6600.030.75661749
CTCGTAT6850.029.63411344
TATGCCG6900.028.91214248
TATCTCG7800.026.47353641
TCGTATG7600.026.2491845
GTATGCC8850.023.33260547
TCTCGTA9500.021.73616643
ACCGCTC9950.019.70029332
AGTCACC10950.019.50093528
CCAGTCA10450.019.09312226
CAGTCAC10850.019.03446427
ATCTCGT11250.018.97718842
GTCACCG10900.018.94807829
CCGTCTT10550.018.90982852
CCGCTCA10450.018.75769633
CACCGCT11200.018.75171331
TGCCGTC11250.018.66608650
GCCGTCT11000.018.4539751
TCCAGTC11350.018.19594225
Produced by FastQC (version 0.11.5)