FastQCFastQC Report
Thu 13 Sep 2018
HGTFWBGX7_n01_EREB117_BECRAFT8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTFWBGX7_n01_EREB117_BECRAFT8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1813980
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT35210.1941035733580304TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC6800.040.14571446
CTCGTAT7850.035.22175244
ATGCCGT8350.033.11266349
TATGCCG8400.032.9155648
TCGTATG9200.030.0533445
TTACTCG9800.029.28796235
TCTCGTA9650.028.2892143
GTATGCC9950.028.13976347
TACTCGA10000.027.65212436
TGCCGTC11350.024.66809850
GCCGTCT11550.024.24094451
ACATTAC11800.024.02660832
CGATCTC11650.023.73376540
ATTACTC11750.023.53307334
GCTTGAA12050.023.23573761
CCGTCTT12050.023.23573752
ACTCGAT12250.022.57129537
GATCTCG12450.022.20870641
CTCGATC13150.021.82496638
ATCTCGT13100.021.3739242
Produced by FastQC (version 0.11.5)