FastQCFastQC Report
Thu 13 Sep 2018
HGTFWBGX7_n01_DBB3_BECRAFT7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTFWBGX7_n01_DBB3_BECRAFT7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2139860
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT3458.7435365E-712.17390144
CGTATGC3953.56702E-711.51897546
AGCGATA4207.8773337E-710.83382934
GCGATAG3401.2261399E-410.29482835
ATGCCGT4005.927896E-59.62476549
TATGCCG4201.01024634E-49.16644348
CACAGCG5957.30375E-68.23586431
CGATAGA4250.00114451348.23586436
ACGTCTG5351.59008E-47.850092415
CATATCG4150.0084776277.5901764
CACGTCT6108.861351E-57.4586614
TCGTATG5904.9072225E-47.11863745
GTCACAG6056.5329246E-46.942628429
ACAGCGA8005.0885756E-56.56264732
GATTGCC5950.00417707636.470581544
TCTCGTA6500.00147625636.461532643
ACACGTC6450.0096595495.968707613
CAGTCAC7050.00367030835.957858627
GATCTCG9407.233623E-55.9575841
GTATGCC7700.00151378745.908946547
Produced by FastQC (version 0.11.5)