FastQCFastQC Report
Thu 13 Sep 2018
HGTFWBGX7_n01_CA3P4_BECRAFT6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTFWBGX7_n01_CA3P4_BECRAFT6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2665205
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC5150.014.27150446
CTCGTAT5950.012.35241544
TATGCCG7050.010.92172548
ATGCCGT6856.548362E-1110.21873449
TCGTATG7201.6370905E-1110.20789845
CCGGCTA6603.601599E-1010.07721833
CGGCTAT7554.2018655E-109.27286834
TCTCGTA8352.8103386E-98.38271943
TCCAGTC9707.8216544E-118.30001725
GGCTATG9554.9294613E-108.06384535
GTATGCC9453.417881E-97.77759247
TCTGAAC10851.03682396E-107.741605318
ACGTCTG9151.5459591E-87.64951715
GTCACCG11001.4006218E-107.63747129
GCTATGA9402.5340341E-87.447748736
ACTCCAG9402.5374902E-87.447190323
AGTCACC11352.764864E-107.402093428
GCTTGAA11352.7830538E-107.40084361
ACCGGCT9251.506196E-77.19009732
ATCTCGT10452.383058E-87.03306142
Produced by FastQC (version 0.11.5)