FastQCFastQC Report
Thu 13 Sep 2018
HGTFWBGX7_n01_BHLH40_BECRAFT1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTFWBGX7_n01_BHLH40_BECRAFT1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2283811
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT4804.360394E-69.47912544
GTCACGA5653.6466918E-79.29301229
CGTATGC4905.6674453E-69.28567346
TCGTATG6301.8438732E-78.8888545
CGAGATT6201.3926583E-68.46826133
ATGCCGT6251.5641072E-68.39996349
TATGCCG5653.4125187E-58.05306248
AGTCACG6653.7855443E-67.895739628
TCACGAG6251.409918E-57.84082430
GAGATTC8101.2575583E-77.778254534
ACTCCAG6754.6887726E-67.777743323
CTCCAGT8251.7121056E-77.63666524
TCTCGTA6703.555764E-57.31324143
AGATTCC7351.5609463E-57.14266935
GCTTGAA8855.53222E-77.118613261
CCGTCTT7956.1822357E-67.04399452
GATTCCA9058.017705E-76.961143536
TCCAGTC8351.2787545E-56.706851525
ACGTCTG8351.2804092E-56.706263515
CACGAGA8702.3395374E-56.43717631
Produced by FastQC (version 0.11.5)