FastQCFastQC Report
Thu 13 Sep 2018
HGTFWBGX7_n01_AtTGA5_col_wg-a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTFWBGX7_n01_AtTGA5_col_wg-a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences830654
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT38880.46806492233830205TruSeq Adapter, Index 7 (97% over 35bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATGCC12600.15168770631333867TruSeq Adapter, Index 7 (96% over 32bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11090.13350925896943852No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT6700.040.22308351
TATGCCG6750.039.92513748
ATGCCGT6850.039.85322649
TGCCGTC7100.037.95699350
GTATGCC7850.034.33053247
CGTATGC8300.032.4692446
CCGTCTT8300.032.4692452
CTGCTTG8350.032.2728759
CTTCTGC9150.029.45296956
GCTTGAA9200.029.29113661
TCGTATG9650.028.28789345
CGTCTTC9700.028.14377253
TTCTGCT10100.027.02754257
TGCTTGA10000.026.94784460
CTCGTAT10450.025.7874144
TCTGCTT11750.023.53003358
AAAGGGG4300.022.79024770
CTTGAAA12100.022.27094762
TCTTCTG12700.021.77122355
TCTCGTA13050.020.64968943
Produced by FastQC (version 0.11.5)