FastQCFastQC Report
Thu 13 Sep 2018
HGTFWBGX7_n01_AtTGA5_col_rr-a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTFWBGX7_n01_AtTGA5_col_rr-a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences886703
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT29690.33483590334080293TruSeq Adapter, Index 6 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATGCC10130.11424343889667679TruSeq Adapter, Index 6 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT4600.034.23901751
ATGCCGT5450.033.39438649
TGCCGTC5550.032.79268650
TATGCCG6100.031.55727648
GTATGCC6550.029.3892247
CGTATGC6550.028.8548746
CCGTCTT6950.026.18696652
CTGCGTG1757.2759576E-1225.99845159
AAAGGGG4500.025.66658470
TGCGTGA2100.024.9999260
TCGTATG7450.024.89924645
CTCGTAT8650.024.27737844
CTGCTTG6850.021.96949459
GCGTGAA2401.8189894E-1221.8749361
TGCTTGA8300.021.50595560
GCTTGAA8200.020.91456661
CTTCTGC8550.020.05841456
TCTCGTA10550.019.90514843
CGTCTTC8700.019.31028253
TCTGCGT2405.020411E-1018.95720358
Produced by FastQC (version 0.11.5)