FastQCFastQC Report
Thu 13 Sep 2018
HGTFWBGX7_n01_AtSPL9_col_wg-a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTFWBGX7_n01_AtSPL9_col_wg-a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences981445
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT35570.3624247920158542TruSeq Adapter, Index 2 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGCC12560.12797456811130528TruSeq Adapter, Index 2 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT6100.044.75126351
TGCCGTC6500.041.46102550
TATGCCG7050.038.2264848
GTATGCC7250.037.1719547
ATGCCGT7450.036.1740549
CGTATGC8350.032.27504746
CCGTCTT8550.031.52007952
CTGCTTG9200.030.05244459
CTTCTGC9900.027.57541756
CTCGTAT9950.027.43684844
TCGTATG9950.027.08509345
GCTTGAA10800.024.95212261
TGCTTGA11300.024.77719360
CGTCTTC11050.024.70557653
TTCTGCT11300.023.84926457
TCTGCTT11600.023.53419158
TCTTCTG13450.021.07780555
TCTCGTA13250.020.60246843
AAAGGGG4650.019.57064870
CTTGAAA14400.019.20115362
Produced by FastQC (version 0.11.5)