FastQCFastQC Report
Thu 13 Sep 2018
HGTFWBGX7_n01_AtSPL9_col_rr-a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTFWBGX7_n01_AtSPL9_col_rr-a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1385859
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT41560.2998862077599525TruSeq Adapter, Index 8 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTATGCC14400.10390667448853022TruSeq Adapter, Index 8 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC7900.041.20102350
GCCGTCT8250.039.02887751
TATGCCG8750.036.39866648
ATGCCGT9200.035.3791449
GTATGCC10000.031.84883547
CCGTCTT10700.030.42054652
CGTATGC10500.030.33222246
CTGCTTG12050.026.43152459
TCGTATG12650.025.17694345
TTCTGCT12900.024.9621357
CTTCTGC13000.024.77011356
CGTCTTC13550.024.2804353
CTCGTAT13200.023.86276444
GCTTGAA14350.022.9259961
TCTGCTT15100.021.55705858
TGCTTGA15700.020.28588160
TCTCGTA16650.019.1284343
TCTTCTG17350.018.76147555
CTTGAAA18950.017.73086262
CATCTCG18600.016.93486441
Produced by FastQC (version 0.11.5)