FastQCFastQC Report
Thu 13 Sep 2018
HGTFWBGX7_n01_AtSPL11_col_wg-a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTFWBGX7_n01_AtSPL11_col_wg-a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences780335
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT30600.3921392735171432TruSeq Adapter, Index 6 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATGCC9490.12161443482606828TruSeq Adapter, Index 6 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8470.10854312570883018No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC4750.042.73824750
ATGCCGT4850.041.8570549
GCCGTCT4950.039.59726751
TATGCCG5400.037.59383448
GTATGCC5850.034.70422447
CCGTCTT6400.033.36047452
CGTATGC6250.032.4831546
CTGCTTG6100.029.26138559
CTTCTGC7550.027.35014356
TCGTATG7250.026.55259945
CGTCTTC8200.024.7537453
CTCGTAT8700.024.13872544
GCTTGAA7850.023.63135161
TCTGCTT8150.023.61889858
TTCTGCT8050.022.6079957
TGCTTGA8250.022.48414860
CTGCGTG1102.9226081E-522.27203659
GCGTGAA1905.620677E-1022.10599361
TCTCGTA9950.021.10622443
TCTTCTG10150.019.99937855
Produced by FastQC (version 0.11.5)