FastQCFastQC Report
Thu 13 Sep 2018
HGTFWBGX7_n01_AtSPL11_col_rr-a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTFWBGX7_n01_AtSPL11_col_rr-a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1078394
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT44890.41626715282169596TruSeq Adapter, Index 6 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTATGCC15260.141506722032949TruSeq Adapter, Index 6 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT7400.037.8366651
TGCCGTC7600.036.84095850
ATGCCGT8250.033.9383449
TATGCCG8300.033.7338948
CTGCTTG9800.029.64330959
CCGTCTT9500.029.47276952
GTATGCC9600.029.16575847
CGTATGC9900.028.28194846
TGCTTGA11550.025.45493160
CGTCTTC11700.023.93087853
TCGTATG11700.023.93087845
CTTCTGC12100.023.42902256
GCTTGAA12350.022.95581661
AAAGGGG5800.022.93138370
CTCGTAT13150.021.82441544
TTCTGCT13400.021.67842957
TCTGCTT13850.020.46962558
TCTTCTG15350.018.69648655
CTTGAAA16550.017.76462262
AAAAGGG7900.016.83491969
Produced by FastQC (version 0.11.5)