FastQCFastQC Report
Thu 13 Sep 2018
HGTFWBGX7_n01_AtLHY_col_wg-a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTFWBGX7_n01_AtLHY_col_wg-a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences770906
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT24480.3175484430008328TruSeq Adapter, Index 1 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTATGCC8420.10922213603214918TruSeq Adapter, Index 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT4200.034.99970651
TGCCGTC4300.034.1857650
ATGCCGT4550.032.30742349
TATGCCG4850.030.30902548
GTATGCC5000.029.39975447
CGTATGC5250.027.99976746
CCGTCTT5700.025.78925952
TCGTATG6200.024.2739945
CGTCTTC6150.023.90223953
CTGCTTG6800.022.13216859
CTTCTGC6650.022.10507856
CTCGTAT6900.021.30416944
TTCTGCT7300.020.61626857
GCTTGAA7350.020.47601961
AAAGGGG4350.020.1160870
TGCTTGA8000.018.81234460
TCTCGTA8550.017.60219243
TCTGCTT8850.016.61003158
TCTTCTG10050.015.67151155
ATCTCGT9500.015.47355642
Produced by FastQC (version 0.11.5)