FastQCFastQC Report
Thu 13 Sep 2018
HGTFWBGX7_n01_AtLHY_col_rr-a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTFWBGX7_n01_AtLHY_col_rr-a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences813917
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT33090.4065525108825593TruSeq Adapter, Index 3 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTATGCC11680.1435035759174461TruSeq Adapter, Index 3 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10650.1308487229041782No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT5100.036.372251
TGCCGTC5300.034.9996650
ATGCCGT5350.034.6725649
TATGCCG5550.033.423148
GTATGCC6100.030.40954247
CCGTCTT6350.029.21231752
CGTATGC6550.028.32033746
CTGCTTG7150.025.45429859
CAGGCGC750.00183267623.33310751
TCGTATG8250.022.9088745
TGCTTGA8450.022.78084660
CTTCTGC8050.022.60847956
GCTTGAA8550.022.10505161
CGTCTTC8400.021.66645653
CTCGTAT8550.021.28634544
TCTGCTT9200.019.78241758
TTCTGCT9450.019.25907357
AAAGGGG3650.018.21900270
TCTTCTG11000.017.18165255
TCTCGTA11200.016.24984443
Produced by FastQC (version 0.11.5)