FastQCFastQC Report
Thu 13 Sep 2018
HGTFWBGX7_n01_AtERF1_col_wg-a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTFWBGX7_n01_AtERF1_col_wg-a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences959088
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT42140.43937574028660564TruSeq Adapter, Index 27 (97% over 39bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCC13390.1396117978746476TruSeq Adapter, Index 27 (97% over 36bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11060.11531788532439151No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG6550.043.2830948
GCCGTCT6600.042.95518551
TGCCGTC6650.042.63221450
ATGCCGT6800.041.691849
GTATGCC7250.039.1040347
CTGCTTG8200.034.99869559
CCGTCTT8850.033.22083352
CGTATGC9000.032.2782646
CTTCTGC9100.031.5389356
CGTCTTC10450.028.13439253
GCTTGAA10500.026.99899361
CTCGTAT11000.026.09129744
TCGTATG11050.025.97323645
TCTGCTT11250.025.82126258
TTCTGCT11150.025.74029457
TGCTTGA11250.025.51016260
TCTTCTG13200.022.2730655
TCTCGTA13150.021.55813243
CTTGAAA13400.021.41711462
TTGAAAA15500.019.6444363
Produced by FastQC (version 0.11.5)