FastQCFastQC Report
Thu 13 Sep 2018
HGTFWBGX7_n01_AtERF1_col_rr-a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTFWBGX7_n01_AtERF1_col_rr-a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences832563
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT38300.46002524733863986TruSeq Adapter, Index 7 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATGCC13200.15854656044047116TruSeq Adapter, Index 7 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8410.10101337676548203No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT7250.047.791351
TGCCGTC7350.046.66490650
ATGCCGT7650.044.8349149
TATGCCG7700.044.54109648
CCGTCTT8750.040.00329652
GTATGCC8650.039.649347
CTGCTTG9150.037.4826759
CGTATGC9350.036.3066146
CTTCTGC9600.035.72996556
CGTCTTC10050.034.8287453
GCTTGAA10550.033.17210861
TCTGCTT10950.031.6445458
TGCTTGA11000.031.49691860
TTCTGCT11050.031.04141657
TCGTATG11000.030.86061745
CTCGTAT10900.030.82267244
CTTGAAA13650.025.89490362
TCTTCTG13850.025.02011155
TCTCGTA13750.024.68997843
TGAAAAA14800.023.64633864
Produced by FastQC (version 0.11.5)