FastQCFastQC Report
Thu 13 Sep 2018
HGTFWBGX7_n01_AtCAMTA1_col_wg-a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTFWBGX7_n01_AtCAMTA1_col_wg-a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1138045
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT53690.47177396324398424TruSeq Adapter, Index 7 (97% over 38bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATGCC18750.16475622668699394TruSeq Adapter, Index 7 (97% over 35bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13460.11827300326437004No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT9050.045.2465251
ATGCCGT9950.040.8021249
TGCCGTC10050.040.39613750
TATGCCG10150.039.99814248
CCGTCTT11100.037.20711552
GTATGCC11150.036.41086647
CTGCTTG11750.034.8509859
CTTCTGC12600.032.50134756
CGTATGC12800.031.99070246
TGCTTGA14000.028.99992860
CGTCTTC14650.028.42996453
GCTTGAA14500.027.9999361
TCGTATG14550.027.90248545
TTCTGCT14950.027.62656257
TCTGCTT15450.026.95904558
CTCGTAT15500.025.96653644
TCTTCTG16950.024.5722155
CTTGAAA18400.022.63680662
TCTCGTA18800.021.5947443
TGAAAAA20100.020.37308564
Produced by FastQC (version 0.11.5)