FastQCFastQC Report
Thu 13 Sep 2018
HGTFWBGX7_n01_AtCAMTA1_col_rr-a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTFWBGX7_n01_AtCAMTA1_col_rr-a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1425788
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT57020.39991920257429575TruSeq Adapter, Index 1 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTATGCC20630.14469191773251003TruSeq Adapter, Index 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT10450.041.5311251
TGCCGTC10550.041.46921550
ATGCCGT10700.040.23366549
TATGCCG11450.037.5982748
GTATGCC12000.035.87501547
CGTATGC13200.032.6136546
CCGTCTT13650.032.3077152
CTGCTTG14150.031.41234259
CGTCTTC15250.029.60657353
CTTCTGC15700.028.53504656
TTCTGCT16400.027.31708757
TGCTTGA16650.026.90597560
TCGTATG16600.026.35543645
GCTTGAA17100.026.19792461
CTCGTAT17050.025.45455744
TCTGCTT17550.025.3267658
TCTTCTG20700.021.81160555
CTTGAAA21800.020.54974762
AAAGGGG8350.019.69991770
TCTCGTA22150.019.43567543
Produced by FastQC (version 0.11.5)