FastQCFastQC Report
Thu 13 Sep 2018
HGTFWBGX7_n01_AtARF2_col_wg-a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTFWBGX7_n01_AtARF2_col_wg-a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1370192
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT60950.4448281700666768TruSeq Adapter, Index 8 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTATGCC20950.15289827994908742TruSeq Adapter, Index 8 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16540.1207130095636232No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT10050.037.61025249
GCCGTCT10200.037.40028451
TATGCCG10150.037.23970848
TGCCGTC10350.036.8582550
GTATGCC11750.032.1687747
CTGCTTG12250.030.85688459
CGTATGC12650.029.88008346
CCGTCTT12950.029.4581452
GCTTGAA14600.026.36964261
TGCTTGA15100.025.96004760
TTCTGCT15100.025.959157
TCGTATG14750.025.62596945
CTTCTGC15350.025.53631456
CGTCTTC15850.024.0683253
TCTGCTT16150.023.83794258
CTCGTAT16200.023.33314144
AAAGGGG7350.020.95220870
TCTTCTG19900.020.04935355
TCTCGTA19550.019.33487943
CTTGAAA20700.018.59887962
Produced by FastQC (version 0.11.5)