FastQCFastQC Report
Thu 13 Sep 2018
HGTFWBGX7_n01_AtARF2_col_rr-a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTFWBGX7_n01_AtARF2_col_rr-a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences780714
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT31490.4033487294963329TruSeq Adapter, Index 19 (97% over 38bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATGCC11470.14691679667586338TruSeq Adapter, Index 19 (97% over 35bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8890.113870124014684No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT6850.040.36178651
TGCCGTC7150.038.67076550
ATGCCGT7250.038.1373749
TATGCCG7950.034.77936648
GTATGCC8000.034.56199347
CGTATGC8400.032.91618346
CCGTCTT8400.032.91407452
CTGCTTG8650.031.96485159
CTTCTGC10000.028.69958156
CGTCTTC9650.028.65243153
TCGTATG9900.028.28241545
TGCTTGA10150.027.58403660
GCTTGAA10300.027.52386961
CTCGTAT10250.026.97521444
TTCTGCT10950.025.2507757
TCTGCTT11250.024.5774258
TCTTCTG12450.023.0518755
TCTCGTA12000.023.03985443
CTTGAAA12400.022.86256862
AAAGGGG4750.022.8403170
Produced by FastQC (version 0.11.5)