FastQCFastQC Report
Wed 8 Aug 2018
HGNJYBGX7_n03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGNJYBGX7_n03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences441706069
Sequences flagged as poor quality0
Sequence length99
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAG104131702.3574885496988722No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGGTCGTGCATTCCGCA54802631.240703577473372No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22318520.5052799036818305No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAGTG19842010.44921298104237734No Hit
GTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAGTGTAT12465410.2822105213138921No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGGGCGGTCGTGCATTCCGCAGC11410500.25832789723339755No Hit
AGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAGTGTA10848420.2456026928622527No Hit
GTGGTATCAACGCAGAGTACATGGGGGGCGGTCGTGCATTCCGCAGCAAC8418450.19058941207348454No Hit
CAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAGTGT8370510.18950407493721805No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGGTCGAGTGCCTCACAGT7845380.1776153997105256No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAGT7725560.17490273605455034No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGTGCATTCCGCAG6693440.15153606594434182No Hit
AGTGGTATCAACGCAGAGTACATGGGGGGCGGTCGTGCATTCCGCAGCAA6590600.14920782082348974No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCGGAACAACTTTTTTATTA5085580.11513493603367264No Hit
CAGTGGTATCAACGCAGAGTACATGGGGGGCGGTCGTGCATTCCGCAGCA5026300.11379286708419667No Hit
GGTGCATATGGTGCCAACCCCCCTGGCCCCCCCAAAAGGGGGGTGGTGCT4650730.10529015393718759No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT79021200.088.3458561
AGCAGTG83013850.083.9662552
GCAGTGG96139600.072.526833
CAGTGGT100601850.069.16764
AGTGGTA108059400.064.4846345
GTGGTAT116799500.059.608616
TGGTATC117358400.059.2283137
GGTATCA120036550.057.976028
GTATCAA142434100.048.8751879
CATGGGA35529600.030.48165524-25
ATCAACG122871650.030.46838610-11
AGAGTAC122572550.030.45016518-19
GCAGAGT123069000.030.40445516-17
TACATGG121807300.030.38986622-23
CAACGCA123228250.030.38350712-13
ACGCAGA123700950.030.31237614-15
AGTACAT123312500.030.13321720-21
CATGGGG76753450.029.99677724-25
TGGGGAG11121950.028.63751826-27
TGGGGGC23154200.028.56204626-27
Produced by FastQC (version 0.11.2)