FastQCFastQC Report
Wed 8 Aug 2018
HGNJYBGX7_n01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGNJYBGX7_n01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences441706069
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGACCT1179500.017.9233361
TTGAACG1429650.017.8881191
GACTAAC1158150.017.8228871
CAATGGA1237600.017.7847658
GGGGGGC517150.017.71313521
GGGAATG1036050.017.6033971
ACGAGGA1091100.017.4546721
CTCGAAA1090200.017.368871
CTGCGGA991900.017.3680841
GGCTCGA1060600.017.3276621
GCGCGAT837800.017.2774561
ACTGATG1090000.017.2053221
CTCGAGG1368700.017.1035921
CGCTGGA1121650.017.090721
ATGCGAT1085800.017.0754721
AGATGCC1257900.017.0679078
TACGGGC1338350.016.9890961
GGAAAGC1012000.016.9649941
TGACTAG1042650.016.952931
CGGACGT1258850.016.9187361
Produced by FastQC (version 0.11.2)